rabbit anti zo1  (Cell Signaling Technology Inc)

Journal: Cell Death Discovery

Article Title: Lysine attenuates acute lung injury by restoring α-tubulin acetylation and ciliary activity

doi: 10.1038/s41420-026-03025-x

Figure Lengend Snippet: A GSEA analysis uncovered homophilic cell adhesion via plasma membrane adhesion molecules enhanced in PQ-injured lung tissues with lysine supplementation compared to untreated group. QPCR analysis of E-Cadherin and Vimentin expression in PQ-injured A549 cells ( B ) and MLE-12 cells ( C ) w/wo lysine supplementation ( n = 3). D WB analysis of ZO-1, E-Cadherin, EPCAM, N-Cadherin, Vimentin, and α-SMA expression in PQ-injured A549 cells w/wo lysine supplementation. E Immunofluorescence analysis of ZO-1 abundance in PQ-poisoned lungs treated w/wo lysine for 3 days. Scale bar, 100 μm. F A549 cells treated w/wo indicated concentration of PQ for 12 h. Wound healing analysis of the injured cells w/wo lysine supplementation for indicated times. G GSEA analysis uncovered cilium movement impaired in PQ-injured lung tissues but restored with lysine supplementation. H Immunofluorescence analysis of ZO-1 and ARL13B in PQ-injured A549 cells w/wo lysine supplementation. Scale bar, 10 μm. I Quantification of percentage of cilia positive cells and cilia length, indicated by ARL13B signal, in PQ-injured A549 cells w/wo lysine supplementation. J WB analysis of acetyl-α-Tubulin in PQ-injured A549 cells w/wo lysine supplementation. Mean ± SD., * P < 0.05, ** P < 0.01, *** P < 0.001; Two-Way ANOVA. NS, not significant.

Article Snippet: Lung microsections (5 μm) were stained with hematoxylin & eosin (H&E), and Masson’s trichrome to visualize fibrotic lesions, or examined by immunofluorescence using Anti-ZO-1 antibody (Cell Signaling Technology, 13663, 1:200).

Techniques: Clinical Proteomics, Membrane, Expressing, Immunofluorescence, Concentration Assay

Journal: Cell Death Discovery

Article Title: Lysine attenuates acute lung injury by restoring α-tubulin acetylation and ciliary activity

doi: 10.1038/s41420-026-03025-x

Figure Lengend Snippet: Calcium image analysis in PQ-injured A549 cells compared to normal cells ( A ) and PQ-injured A549 cells w/wo lysine supplementation ( B ) with thapsigargin (TG) or ionomycin (IONO) stimulation. Mean ± SEM. C NFAT luciferase expression in A549 cells treated w/wo 800 μM PQ, together with 0-, 1-, 2.5-, or 5-fold lysine as in the culture medium as indicated, for 24 h. Mean ± SD, *** P < 0.001; Two-Way ANOVA. n = 3. NS not significant. D WB analysis of E-Cadherin, ZO-1, EPCAM and acetyl-α-Tubulin in PQ-injured A549 cells treated w/wo 5 μM SKF-96365 (SKF). E Co-IP analysis of STIM1 association with ORAI1 or TRPC1 in PQ-injured A549 cells with indicated concentration, together w/wo 5 mM lysine. F Immunofluorescence analysis of Myc and ARL13B in PQ-injured TRPC1-Myc stably expressed A549 cells w/wo lysine supplementation. Scale bar, 10 μm.

Article Snippet: Lung microsections (5 μm) were stained with hematoxylin & eosin (H&E), and Masson’s trichrome to visualize fibrotic lesions, or examined by immunofluorescence using Anti-ZO-1 antibody (Cell Signaling Technology, 13663, 1:200).

Techniques: Luciferase, Expressing, Co-Immunoprecipitation Assay, Concentration Assay, Immunofluorescence, Stable Transfection

Journal: Cell Death Discovery

Article Title: Lysine attenuates acute lung injury by restoring α-tubulin acetylation and ciliary activity

doi: 10.1038/s41420-026-03025-x

Figure Lengend Snippet: A GSEA analysis uncovered top-ranked molecular functions enhanced in PQ-injured lung tissues with lysine supplementation compared to the untreated group. B KEGG enrichment of the top 20 upregulated pathways in PQ-injured lung tissues with lysine supplementation compared to the untreated group. C Quantification of NADP and NADPH (left) or NAD and NADH (right) in PQ-injured A549 cells w/wo lysine supplementation. D ELISA analysis of acetyl coenzyme A (Acetyl-CoA) in PQ-injured A549 cells w/wo lysine supplementation. E WB analysis of E-Cadherin and acetyl-α-Tubulin in PQ-injured A549 cells treated w/wo 1 mM sodium acetate (SA). F WB analysis of ZO-1, E-Cadherin, acetyl-α-Tubulin and EPCAM in PQ-injured A549 cells w/wo lysine supplementation, together w/wo 0.5 μM GM-90257 (GM). G Calcium image analysis in PQ-injured A549 cells w/wo lysine supplementation, together w/wo 0.5 μM GM-90257 treatment. The cells were transiently stimulated with thapsigargin (TG) or ionomycin (IONO). Mean ± SEM. H Co-IP analysis of STIM1 association with TRPC1 in PQ-injured A549 cells w/wo lysine supplementation, together w/wo GM-90257 treatment. Immunofluorescence analysis of ARL13B, SFTPC, and HOPX in A549 cells ( I ) or normal lung tissues ( J ). Scale bar, 10 μm. Mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001; Two-Way ANOVA. NS not significant.

Article Snippet: Lung microsections (5 μm) were stained with hematoxylin & eosin (H&E), and Masson’s trichrome to visualize fibrotic lesions, or examined by immunofluorescence using Anti-ZO-1 antibody (Cell Signaling Technology, 13663, 1:200).

Techniques: Enzyme-linked Immunosorbent Assay, Co-Immunoprecipitation Assay, Immunofluorescence